pGEM-HE-h_bPAC_cmyc
(Plasmid #28134)

• Purpose: optogenetic tool for light modulation of cellular cAMP
• Depositing Lab: Peter Hegemann
• Publication: Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

Backbone

• Vector backbone: pGEM-HE
• Backbone size w/o insert (bp): 3000
• Vector type: in vitro transcription

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: humanized photoactivated adenylyl cyclase
• Alt name: bPAC
• Alt name: BlaC
• Species: Beggiatoa sp. PS
• Insert Size (bp): 1050
• GenBank ID: GU461306
• Tag / Fusion Protein:
  • Myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SplI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6, M13

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pGEM-HE is a derivative of pGEM3Z vector(Promega) modified after
Liman, E.R., Tytgat, J. & Hess,P.; Neuron 9, 1992