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pET28a-ec_bPAC
(Plasmid #28135)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28135 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    E.coli adapted photoactivated adenylyl cyclase
  • Alt name
    bPAC
  • Alt name
    BlaC
  • Species
    Beggiatoa sp. PS
  • Insert Size (bp)
    1050
  • GenBank ID
    GU461307
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7, M13_fw
  • 3′ sequencing primer M13_ter
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

PLEASE NOTE: The codon usage of this gene is optimized for expression in
E.coli. The humanized version for expression in neurons is Plasmid #28134.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-ec_bPAC was a gift from Peter Hegemann (Addgene plasmid # 28135)
  • For your References section:

    Light modulation of cellular cAMP by a small bacterial photoactivated adenylyl cyclase, bPAC, of the soil bacterium Beggiatoa. Stierl M, Stumpf P, Udwari D, Gueta R, Hagedorn R, Losi A, Gartner W, Petereit L, Efetova M, Schwarzel M, Oertner TG, Nagel G, Hegemann P. J Biol Chem. 2011 Jan 14. 286(2):1181-8. 10.1074/jbc.M110.185496 PubMed 21030594