Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||28184||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameAdcy4 promoter
SpeciesM. musculus (mouse)
Insert Size (bp)1812
Entrez GeneAdcy4 (a.k.a. KIAA4004, mKIAA4004)
/ Fusion Protein
- Luciferase (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer Luc_N_Rev (Common Sequencing Primers)
Contains a total of ~1812 bp of genomic DNA--i.e.,1459 bp upstream of the Adcy4 transcription start site and 353 bp downstream of the transcription start site. The downstream DNA includes exon I, intron I and the non-coding portion of exon II.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p-1459-adcy4-Luc was a gift from Bernard Schimmer (Addgene plasmid # 28184 ; http://n2t.net/addgene:28184 ; RRID:Addgene_28184)
For your References section:A polymorphic form of steroidogenic factor-1 is associated with adrenocorticotropin resistance in y1 mouse adrenocortical tumor cell mutants. Frigeri C, Tsao J, Cordova M, Schimmer BP. Endocrinology. 2002 Oct . 143(10):4031-7. 10.1210/en.2002-220349 PubMed 12239114