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p-1459-adcy4-Luc
(Plasmid #28184)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28184 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Adcy4 promoter
  • Alt name
    Adcy4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1812
  • Entrez Gene
    Adcy4 (a.k.a. KIAA4004, mKIAA4004)
  • Tag / Fusion Protein
    • Luciferase (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

Contains a total of ~1812 bp of genomic DNA--i.e.,1459 bp upstream of the Adcy4 transcription start site and 353 bp downstream of the transcription start site. The downstream DNA includes exon I, intron I and the non-coding portion of exon II.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-1459-adcy4-Luc was a gift from Bernard Schimmer (Addgene plasmid # 28184 ; http://n2t.net/addgene:28184 ; RRID:Addgene_28184)
  • For your References section:

    A polymorphic form of steroidogenic factor-1 is associated with adrenocorticotropin resistance in y1 mouse adrenocortical tumor cell mutants. Frigeri C, Tsao J, Cordova M, Schimmer BP. Endocrinology. 2002 Oct . 143(10):4031-7. 10.1210/en.2002-220349 PubMed 12239114