SIRT6
(Plasmid
#28271)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 28271 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28a-LIC (Addgene plasmid 25353)
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Backbone manufacturerSGC
- Backbone size w/o insert (bp) 7328
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSIRT6
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Alt namePDB: 3PKJ
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Alt nameNAD-dependent deacetylase sirtuin-6
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SpeciesH. sapiens (human)
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Insert Size (bp)1065
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Entrez GeneSIRT6 (a.k.a. SIR2L6)
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Tag
/ Fusion Protein
- His tag with thrombin cleavage (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site LIC (unknown if destroyed)
- 3′ cloning site LIC (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Article Citing this Plasmid
Depositor Comments
PDB: 3PKJ. http://www.thesgc.org/structures/3PKJ/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SIRT6 was a gift from Cheryl Arrowsmith (Addgene plasmid # 28271 ; http://n2t.net/addgene:28271 ; RRID:Addgene_28271)