SIRT6
(Plasmid #28271)

• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC Q4 (unpublished)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	28271	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pET28a-LIC (Addgene plasmid 25353)
  (Search Vector Database)
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7328
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SIRT6
• Alt name: PDB: 3PKJ
• Alt name: NAD-dependent deacetylase sirtuin-6
• Species: H. sapiens (human)
• Insert Size (bp): 1065
• Entrez Gene: SIRT6 (a.k.a. SIR2L6)
• Tag / Fusion Protein:
  • His tag with thrombin cleavage (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: LIC (unknown if destroyed)
• 3′ cloning site: LIC (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Terms and Licenses:
  • UBMTA
• Industry Terms:
  • Not Available to Industry
• Article Citing this Plasmid:
  • 1 Reference

Depositor Comments

PDB: 3PKJ. http://www.thesgc.org/structures/3PKJ/