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pCMV-PTEN-C124S
(Plasmid #28300)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28300 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PTEN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1209
  • Mutation
    Phosphatase Inactive Mutant Cys124Ser
  • Entrez Gene
    PTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-PTEN-C124S was a gift from Maria-Magdalena Georgescu (Addgene plasmid # 28300 ; http://n2t.net/addgene:28300 ; RRID:Addgene_28300)
  • For your References section:

    PTEN tumor suppressor associates with NHERF proteins to attenuate PDGF receptor signaling. Takahashi Y, Morales FC, Kreimann EL, Georgescu MM. EMBO J. 2006 Feb 22. 25(4):910-20. 10.1038/sj.emboj.7600979 PubMed 16456542