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pEMS1503
(Plasmid #29299)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 29299 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEMS1313
  • Backbone size w/o insert (bp) 18843
  • Vector type
    Pleiades Promoter Project [sic, Pleaides Plieades]

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ple32
  • Alt name
    CLDN5
  • Alt name
    Claudin 5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1670
  • Entrez Gene
    CLDN5 (a.k.a. AWAL, BEC1, CPETRL1, TMDVCF, TMVCF)
  • Tag / Fusion Protein
    • Intron-LacZ-NLS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site FseI (unknown if destroyed)
  • 3′ cloning site AscI (unknown if destroyed)
  • 5′ sequencing primer Unknown
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMS1503 was a gift from Elizabeth Simpson (Addgene plasmid # 29299 ; http://n2t.net/addgene:29299 ; RRID:Addgene_29299)
  • For your References section:

    Targeted CNS Delivery Using Human MiniPromoters and Demonstrated Compatibility with Adeno-Associated Viral Vectors. de Leeuw CN, Dyka FM, Boye SL, Laprise S, Zhou M, Chou AY, Borretta L, McInerny SC, Banks KG, Portales-Casamar E, Swanson MI, D'Souza CA, Boye SE, Jones SJ, Holt RA, Goldowitz D, Hauswirth WW, Wasserman WW, Simpson EM. Mol Ther Methods Clin Dev. 2014 Jan 8;1:5. 10.1038/mtm.2013.5 PubMed 24761428