This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Lenti-sh1368 knockdown c-myc
(Plasmid #29435)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 29435 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    FUGW, modified
  • Backbone manufacturer
    David Baltimore (Caltech) and Valerie
  • Backbone size w/o insert (bp) 7000
  • Vector type
    Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Growth instructions
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-Myc shRNA
  • Alt name
    c-Myc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    60
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Tags / Fusion Proteins
    • GFP (N terminal on backbone)
    • WPRE (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

sh1368 sequence= GACGAGAACAGTTGAAACA

The FUGW modified vector was obtained from Dr. Valera Vasioukhin at FHCRC (Lien et al., 2008).

To generate the lentiviral vector for production of shRNA, the FUGW vector (provided by D. Baltimore, California Institute of Technology, Pasadena, CA; Lois et al., 2002) was modified by placing internal ribosome entry site–neomycin sequences downstream from EGFP and cloning of human H1 promoter followed by unique BamHI–EcoRI sites upstream from the ubiquitin promoter. Please note that the insert was cloned into BamHI-EcoRI sites using BglII-EcoRI fragment. The BamHI site was destroyed after ligation.

See the attached document for a map from Lien et al., 2008 for vector information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-sh1368 knockdown c-myc was a gift from Bob Eisenman (Addgene plasmid # 29435 ; http://n2t.net/addgene:29435 ; RRID:Addgene_29435)
  • For your References section:

    Myc-regulated microRNAs attenuate embryonic stem cell differentiation. Lin CH, Jackson AL, Guo J, Linsley PS, Eisenman RN. EMBO J. 2009 Oct 21. 28(20):3157-70. 10.1038/emboj.2009.254 PubMed 19745813