Lenti-sh1368 knockdown c-myc
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||29435||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneFUGW, modified
Backbone manufacturerDavid Baltimore (Caltech) and Valerie
- Backbone size w/o insert (bp) 7000
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namec-Myc shRNA
SpeciesH. sapiens (human)
Insert Size (bp)60
Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
/ Fusion Proteins
- GFP (N terminal on backbone)
- WPRE (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-Fwd (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
sh1368 sequence= GACGAGAACAGTTGAAACA
The FUGW modified vector was obtained from Dr. Valera Vasioukhin at FHCRC (Lien et al., 2008).
To generate the lentiviral vector for production of shRNA, the FUGW vector (provided by D. Baltimore, California Institute of Technology, Pasadena, CA; Lois et al., 2002) was modified by placing internal ribosome entry site–neomycin sequences downstream from EGFP and cloning of human H1 promoter followed by unique BamHI–EcoRI sites upstream from the ubiquitin promoter. Please note that the insert was cloned into BamHI-EcoRI sites using BglII-EcoRI fragment. The BamHI site was destroyed after ligation.
See the attached document for a map from Lien et al., 2008 for vector information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Lenti-sh1368 knockdown c-myc was a gift from Bob Eisenman (Addgene plasmid # 29435 ; http://n2t.net/addgene:29435 ; RRID:Addgene_29435)
For your References section:Myc-regulated microRNAs attenuate embryonic stem cell differentiation. Lin CH, Jackson AL, Guo J, Linsley PS, Eisenman RN. EMBO J. 2009 Oct 21. 28(20):3157-70. 10.1038/emboj.2009.254 PubMed 19745813