Addgene - AP-SEMA3A (chick) Plasmid Data

Plasmid 29448: AP-SEMA3A (chick)

Gene/insert name:
AP-SEMA3A (chick)
Alt name:
AP-Collapsin1
Alt name:
Collapsin1
Alt name:
Semaphorin 3A
Insert size:
3800
Species:
G. gallus (chicken)
GenBank ID:
NM_204977
Entrez Gene:
SEMA3A (COLL-1)
Fusion protein or tag:
AP tagged
Terminal:
N terminal on insert
Vector backbone:
pAG
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6000
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
Xho1
Site destroyed during cloning:
No
5' sequencing primer:
TTC GGC TTC TGG CGT GTG ACC List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Jonathan Raper
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Secreted chick semaphorins bind recombinant neuropilin with similar affinities but bind different subsets of neurons in situ. Feiner et al (Neuron. 1997 Sep . 19(3):539-45. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 29448" in your Materials and Methods section.