Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at Learn more

AP-SEMA3A (chick)
(Plasmid #29448)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 29448 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    AP-SEMA3A (chick)
  • Alt name
  • Alt name
  • Alt name
    Semaphorin 3A
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    SEMA3A (a.k.a. COLL-1)
  • Tag / Fusion Protein
    • AP tagged (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer TTC GGC TTC TGG CGT GTG ACC
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AP-SEMA3A (chick) was a gift from Jonathan Raper (Addgene plasmid # 29448)
  • For your References section:

    Secreted chick semaphorins bind recombinant neuropilin with similar affinities but bind different subsets of neurons in situ. Feiner L, Koppel AM, Kobayashi H, Raper JA. Neuron. 1997 Sep . 19(3):539-45. 10.1016/S0896-6273(00)80370-0 PubMed 9331347