Addgene - pSpy Plasmid Data

Plasmid 29588: pSpy

Gene/insert name:
Spy
Alt name:
spheroplast protein Y
Alt name:
envelope stress induced periplasmic protein
Insert size:
420
Species:
E.coli BL21(DE3)
GenBank ID:
AM946981
Entrez Gene:
spy (ECK1741, JW1732)
Mutation:
processed form of Spy starting at residue 24 (Removal of first 23aa of periplasmic sequence)
Vector backbone:
pLysE
(Search Vector Database)
Backbone manufacturer:
Novagen
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
4430
5' sequencing primer:
pBR2 List of Sequencing Primers
3' sequencing primer:
pBR3
Bacterial resistance(s)
Chloramphenicol
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (1)
Principal Investigator:
Marc Fiedler
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 29588" in your Materials and Methods section.

Price: $65.00

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