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pET empty polycistronic destination vector (2Z)
(Plasmid #29776)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site several (destroyed during cloning)
  • 3′ cloning site several (destroyed during cloning)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid is an empty destination vector. It is a polycistronic vector that can express up to five genes at once. Genes must first be cloned into any of our 2-series transfer vectors, then subcloned into any of the five cassettes of the destination vector:

Cassette 1: BamHI/XbaI
Cassette 2: AsiSI/PspXI
Cassette 3: SbfI/AscI
Cassette 4: AdeI/NotI
Cassette 5: PacI/FseI

Please note that you must always insert your gene into the lowest cassette number first (e.g., if you're using cassettes 2 and 3, you must put your gene into cassette 2 first, then move on to cassette 3). You do not have to use all of the cassettes.

More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET empty polycistronic destination vector (2Z) was a gift from Scott Gradia (Addgene plasmid # 29776)