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pGEX-2T-IQGAP1
(Plasmid #30107)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 30107 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-2T
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4948
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IQGAP1
  • Species
    H. sapiens (human)
  • Entrez Gene
    IQGAP1 (a.k.a. HUMORFA01, SAR1, p195)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-2T-IQGAP1 was a gift from David Sacks (Addgene plasmid # 30107 ; http://n2t.net/addgene:30107 ; RRID:Addgene_30107)
  • For your References section:

    IQGAP1 integrates Ca2+/calmodulin and Cdc42 signaling. Ho YD, Joyal JL, Li Z, Sacks DB. J Biol Chem. 1999 Jan 1. 274(1):464-70. 10.1074/jbc.274.1.464 PubMed 9867866