|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||30107||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4948
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneIQGAP1 (a.k.a. HUMORFA01, SAR1, p195)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pGEX5' (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEX-2T-IQGAP1 was a gift from David Sacks (Addgene plasmid # 30107 ; http://n2t.net/addgene:30107 ; RRID:Addgene_30107)
For your References section:IQGAP1 integrates Ca2+/calmodulin and Cdc42 signaling. Ho YD, Joyal JL, Li Z, Sacks DB. J Biol Chem. 1999 Jan 1. 274(1):464-70. 10.1074/jbc.274.1.464 PubMed 9867866