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pEGFP-IQGAP1
(Plasmid #30112)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 30112 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IQGAP1
  • Species
    H. sapiens (human)
  • Entrez Gene
    IQGAP1 (a.k.a. HUMORFA01, SAR1, p195)
  • Promoter CMV
  • Tag / Fusion Protein
    • tandem EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-IQGAP1 was a gift from David Sacks (Addgene plasmid # 30112 ; http://n2t.net/addgene:30112 ; RRID:Addgene_30112)
  • For your References section:

    Self-association of IQGAP1: characterization and functional sequelae. Ren JG, Li Z, Crimmins DL, Sacks DB. J Biol Chem. 2005 Oct 14. 280(41):34548-57. 10.1074/jbc.M507321200 PubMed 16105843