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Plasmid 30321: pLKO.1 RagD shRNA #1
  • RagD shRNA

  • RagD

  • H. sapiens (human)

  • RRAGD (RAGD, bA11D8.2.1)

  • pLKO.1 puro
    (Search Vector Database)

  • Available at Addgene (#8453)

  • Mammalian Expression, Lentiviral, RNAi

  • 7000

  • AgeI

  • Unknown

  • EcoRI

  • Unknown

  • LKO.1 5' List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • High Copy

  • Puromycin

  • View sequences (1)
  • David Sabatini

  • UBMTA

Comments: 

shRNA directed against RagD. Target sequence: 5'-CGGCAAGTCGTCTATTCAGAA-3'. Sequence from the website of the RNAi consortium at the Broad institute.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al (Science. 2008 Jun 13. 320(5882):1496-501. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 30321" in your Materials and Methods section.