- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
SpeciesH. sapiens (human)
MutationCatalytically inactive mutant (H216/611A)
Entrez GeneHDAC6 (a.k.a. CPBHM, HD6, JM21, PPP1R90)
/ Fusion Protein
- FLAG (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA-HDAC6.DC-FLAG was a gift from Tso-Pang Yao (Addgene plasmid # 30483)
For your References section:The deacetylase HDAC6 regulates aggresome formation and cell viability in response to misfolded protein stress. Kawaguchi Y, Kovacs JJ, McLaurin A, Vance JM, Ito A, Yao TP. Cell. 2003 Dec 12. 115(6):727-38. 10.1016/S0092-8674(03)00939-5 PubMed 14675537
Map generated by Addgene from plasmid data.