pcDNA-HDAC6.DC-FLAG
(Plasmid #30483)

Loading...
Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    HDAC6
  • Species
    H. sapiens (human)
  • Mutation
    Catalytically inactive mutant (H216/611A)
  • Entrez Gene
    HDAC6 (a.k.a. JM21, CPBHM, HD6, PPP1R90)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-HDAC6.DC-FLAG was a gift from Tso-Pang Yao (Addgene plasmid # 30483)
  • For your References section:

    The deacetylase HDAC6 regulates aggresome formation and cell viability in response to misfolded protein stress. Kawaguchi Y, Kovacs JJ, McLaurin A, Vance JM, Ito A, Yao TP. Cell. 2003 Dec 12. 115(6):727-38. 10.1016/S0092-8674(03)00939-5 PubMed 14675537