Vector backbonepCR8-GW modified
- Backbone size w/o insert (bp) 2676
Growth in Bacteria
Bacterial Resistance(s)Ampicillin (50 ug/ml)
Copy numberHigh Copy
Insert Size (bp)2101
- Cloning method Restriction Enzyme
- 5′ sequencing primer TAL_F1 (ttggcgtcggcaaacagtgg)
- 3′ sequencing primer TAL_R2 (ggcgacgaggtggtcgttgg) (Common Sequencing Primers)
Gateway compatible vector for assembly of full-length TAL effector genes by Golden Gate cloning. Contains both SD and Kozak sequences immediately upstream of the initial ATG. Designed for TAL DNA-binding domains alone
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTAL1 was a gift from Adam Bogdanove & Daniel Voytas (Addgene plasmid # 31031)
For your References section:Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687
Map generated by Addgene from full sequence supplied by depositor.