pTAL1
(Plasmid #31031)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCR8-GW modified
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2676

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin (50 ug/ml)
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH10B/LB+antibiotics
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    TALE
  • Species
    Xanthamonas oryzae
  • Insert Size (bp)
    2101

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer TAL_F1 (ttggcgtcggcaaacagtgg)
  • 3′ sequencing primer TAL_R2 (ggcgacgaggtggtcgttgg)
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Gateway compatible vector for assembly of full-length TAL effector genes by Golden Gate cloning. Contains both SD and Kozak sequences immediately upstream of the initial ATG. Designed for TAL DNA-binding domains alone

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTAL1 was a gift from Adam Bogdanove & Daniel Voytas (Addgene plasmid # 31031)
  • For your References section:

    Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687