Addgene - pTAL1 Plasmid Data

Plasmid 31031: pTAL1

Gene/insert name:
TALE
Insert size:
2101
Species:
Xanthamonas oryzae
Vector backbone:
pCR8-GW modified
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Backbone size w/o insert (bp):
2676
5' sequencing primer:
TAL_F1 (ttggcgtcggcaaacagtgg) List of Sequencing Primers
3' sequencing primer:
TAL_R2 (ggcgacgaggtggtcgttgg)
Bacterial resistance(s)
Ampicillin (50 ug/ml)
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
DH10B/LB+antibiotics
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Adam Bogdanove
Principal Investigator:
Daniel Voytas
Terms and Licenses:
MTA
Cellectis Limited Use Label License

Comments:

Gateway compatible vector for assembly of full-length TAL effector genes by Golden Gate cloning. Contains both SD and Kozak sequences immediately upstream of the initial ATG. Designed for TAL DNA-binding domains alone

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak et al (Nucleic Acids Res. 2011 Apr 14. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31031" in your Materials and Methods section.