Addgene - pTAL3 Plasmid Data

Plasmid 31034: pTAL3

Gene/insert name:
TALEN-FokI nuclease backbone
Insert size:
1948
Species:
Xanthamonas oryzae
Fusion protein or tag:
FokI nuclease
Terminal:
C terminal on insert
Fusion protein or tag:
AcV5
Vector backbone:
pDW1789
(Search Vector Database)
Backbone size w/o insert (bp):
5582
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
TEF_seq (ggtcttcaatttctcaagtttc) TAL_F1 (ttggcgtcggcaaacagtgg) List of Sequencing Primers
3' sequencing primer:
homodimer_rev (aattcagatttcactagctg) TAL_R2 (ggcgacgaggtggtcgttg
Bacterial resistance(s)
Ampicillin (50 ug/ml)
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
DH5a/LB+antibiotics
High or low copy:
High Copy
Selectable markers:
HIS3
Sequence: View sequences (7)
Principal Investigator:
Adam Bogdanove
Principal Investigator:
Daniel Voytas
Terms and Licenses:
MTA
Cellectis Limited Use Label License

Comments:

Expression of the TAL-FokI homodimer in yeast.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak et al (Nucleic Acids Res. 2011 Apr 14. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31034" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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