Addgene - HNF4A_P2-135 Plasmid Data

Plasmid 31067: HNF4A_P2-135

Gene/insert name:
HNF4A P2 promoter
Insert size:
135
Species:
H. sapiens (human)
Entrez Gene:
HNF4A (RP5-1013A22.1, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14)
Mutation:
-135 to -1 of P2 HNF4A promoter
Vector backbone:
pGL3-basic
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
4800
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Gerhart Ryffel
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A distant upstream promoter of the HNF-4alpha gene connects the transcription factors involved in maturity-onset diabetes of the young. Thomas et al (Hum Mol Genet. 2001 Sep 15. 10(19):2089-97. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31067" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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