pcDNA3.1-Myc-His RTN3A1
(Plasmid #31087)

• Depositing Lab: Jimtong Horng
• Publication: Tang et al J Biol Chem. 2007 Feb 23. 282(8):5888-98.

Backbone

• Vector backbone: pcDNA3.1/myc-His(-)B (invitrogen)
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: reticulon 3
• Alt name: RTN3
• Species: H. sapiens (human)
• Insert Size (bp): 711
• GenBank ID: AF059524
• Entrez Gene: RTN3 (a.k.a. ASYIP, HAP, NSPL2, NSPLII, RTN3-A1)
• Tag / Fusion Protein:
  • Myc-His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry