Addgene - OPN(-213)-luc Plasmid Data

Plasmid 31107: OPN(-213)-luc

Gene/insert name:
osteopontin promoter
Alt name:
SPP1
Insert size:
300
Species:
H. sapiens (human)
GenBank ID:
NM_000582
Entrez Gene:
SPP1 (PSEC0156, BNSP, BSPI, ETA-1, OPN)
Mutation:
promoter region -213 to +87
Vector backbone:
pGL3-Basic
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
4800
Cloning site 5':
SacI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Gerhart Ryffel
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

promoter luciferase reporter

Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Identification of target genes of the transcription factor HNF1beta and HNF1alpha in a human embryonic kidney cell line. Senkel et al (Biochim Biophys Acta. 2005 Dec 20. 1731(3):179-90. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31107" in your Materials and Methods section.