Depositor Sequences: None.
Addgene Sequences: Partial (2)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31167||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeExpression vector
Growth in Bacteria
Alt nameviral 2A peptide
Mutationmembrane tethered Akt
/ Fusion Proteins
- Myr (N terminal on insert)
- mCherry (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Expression clone for generating mRNA encoding membrane tethered Akt in frame with the viral 2A peptide and mCherry.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:786 pCSmAKT2Acherry was a gift from Nathan Lawson (Addgene plasmid # 31167)
For your References section:A genetic screen for vascular mutants in zebrafish reveals dynamic roles for Vegf/Plcg1 signaling during artery development. Covassin LD, Siekmann AF, Kacergis MC, Laver E, Moore JC, Villefranc JA, Weinstein BM, Lawson ND. Dev Biol. 2009 May 15. 329(2):212-26. 10.1016/j.ydbio.2009.02.031 PubMed 19269286
Generated by Addgene from plasmid data.