Addgene - 786 pCSmAKT2Acherry Plasmid Data

Plasmid 31167: 786 pCSmAKT2Acherry

Gene/insert name:
Akt
Alt name:
viral 2A peptide
Fusion protein or tag:
Myr
Terminal:
N terminal on insert
Fusion protein or tag:
mCherry
Terminal:
C terminal on insert
Mutation:
membrane tethered Akt
Vector backbone:
pCS2+
(Search Vector Database)
Vector type:
Expression vector
Backbone size w/o insert (bp):
5000
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
EBV-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Nathan Lawson
Terms and Licenses:
MTA
Clontech Limited Use Label License

Comments:

Expression clone for generating mRNA encoding membrane tethered Akt in frame with the viral 2A peptide and mCherry.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A genetic screen for vascular mutants in zebrafish reveals dynamic roles for Vegf/Plcg1 signaling during artery development. Covassin et al (Dev Biol. 2009 May 15. 329(2):212-26. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31167" in your Materials and Methods section.