Depositor Sequences: None.
Addgene Sequences: Partial (2)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31880||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeExpression Vector
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationSer218Glu Ser222Asp; activated MEKK
Entrez GeneMAP2K1 (a.k.a. CFC3, MAPKK1, MEK1, MKK1, PRKMK1)
- Promoter CMV
/ Fusion Protein
- mCherry (N terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-Fwd
- 3′ sequencing primer EBV-Rev (Common Sequencing Primers)
Expression clone for generating mRNA encoding mcherry-actMEKK.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:783 pCScherryActMEK was a gift from Nathan Lawson (Addgene plasmid # 31880)
For your References section:A genetic screen for vascular mutants in zebrafish reveals dynamic roles for Vegf/Plcg1 signaling during artery development. Covassin LD, Siekmann AF, Kacergis MC, Laver E, Moore JC, Villefranc JA, Weinstein BM, Lawson ND. Dev Biol. 2009 May 15. 329(2):212-26. 10.1016/j.ydbio.2009.02.031 PubMed 19269286
Generated by Addgene from plasmid data.