Addgene - HA-Akt DN (K179M) Plasmid Data

Plasmid 16243: HA-Akt DN (K179M)

Gene/insert name:
Akt1
Species:
M. musculus (mouse)
Entrez Gene:
Akt1 (Akt, PKB, PKB/Akt, PKBalpha, Rac)
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Mutation:
Dominant negative (K179M)
Vector backbone:
pCMV5
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
HindIII
Site destroyed during cloning:
Unknown
Cloning site 3':
BamHI
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Dr. Philip Tsichlis
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Mien-Chie Hung
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: HER-2/neu blocks tumor necrosis factor-induced apoptosis via the Akt/NF-kappaB pathway. Zhou et al (J Biol Chem. 2000 Mar 17. 275(11):8027-31. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16243" in your Materials and Methods section.