Addgene - CLuc-FKBP Plasmid Data

Plasmid 31184: CLuc-FKBP

Gene/insert name:
C-terminus of Firefly Luciferase and FK506-binding protein 12
Alt name:
FKBP
Alt name:
FLUC
Insert size:
862
Species:
H. sapiens (human)
Vector backbone:
pEF6-V5/HIS TOPO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5840
Cloning method:
TOPO Cloning
5' sequencing primer:
Unknown List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Blasticidin
Sequence: View sequences (3)
Principal Investigator:
David Piwnica-Worms
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Kinetics of regulated protein-protein interactions revealed with firefly luciferase complementation imaging in cells and living animals. Luker et al (Proc Natl Acad Sci U S A. 2004 Aug 17. 101(33):12288-93. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31184" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with