Addgene - pLenti6/V5-DEST-MCM7 Plasmid Data

Plasmid 31212: pLenti6/V5-DEST-MCM7

Gene/insert name:
MCM7
Insert size:
2160
Species:
H. sapiens (human)
GenBank ID:
BC013375
Entrez Gene:
MCM7 (CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146)
Fusion protein or tag:
V5
Terminal:
C terminal on backbone
Vector backbone:
pLenti6/V5-DEST
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
7000
Cloning method:
Gateway Cloning
Cloning site 5':
attR1
Site destroyed during cloning:
Yes
Cloning site 3':
attR2
Site destroyed during cloning:
Yes
5' sequencing primer:
CMV Forward List of Sequencing Primers
3' sequencing primer:
V5 Reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
30
Growth instructions:
Grow in STBL3 bacteria (Invitrogen) at lower temps (~30C) to prevent recombination and increase plasmid production.
High or low copy:
High Copy
Selectable markers:
Blasticidin
Person or lab that originally
cloned the gene/insert:
The entry clone used to create this plasmid was obatined from the human ORFeome (v5.1) collection made available by the Center for Cancer Systems Biology at the Dana-Farber Cancer Institute in Boston, MA.
Sequence: View sequences (1)
Principal Investigator:
Lynda Chin
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Proinvasion metastasis drivers in early-stage melanoma are oncogenes. Scott et al (Cancer Cell. 2011 Jul 12;20(1):92-103. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31212" in your Materials and Methods section.