pEFX-AP
(Plasmid
#31234)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31234 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEFX (modified pEF1/myc-His C)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAP
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Alt namealkaline phosphatase
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SpeciesH. sapiens (human)
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Entrez GeneALPP (a.k.a. ALP, ALPI, IAP, PALP, PLAP, PLAP-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer EF1a-Fwd
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pEF1/myc-His C (Invitrogen) was modified to make pEFX by excising a 2.2kb PvuII fragment containing Neomycin and SV40 elements.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEFX-AP was a gift from Cliff Ragsdale (Addgene plasmid # 31234 ; http://n2t.net/addgene:31234 ; RRID:Addgene_31234) -
For your References section:
Sonic hedgehog control of size and shape in midbrain pattern formation. Agarwala S, Sanders TA, Ragsdale CW. Science. 2001 Mar 16. 291(5511):2147-50. 10.1126/science.1058624 PubMed 11251119