pCARCre
(Plasmid #31309)

• Depositing Lab: Gerhart Ryffel
• Publication: Ryffel et al Nucleic Acids Res. 2003 Apr 15. 31(8):e44.

Backbone

• Vector backbone: N/A
• Backbone size w/o insert (bp): 3000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cardiac actin / Cre
• Species: X. laevis (frog); bateriophage P1
• Insert Size (bp): 4500

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pCARGFP (Kroll et al 1996) used to replace the GFP with Cre from pCSCre2