|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17149||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeXenopus expression
Growth in Bacteria
Gene/Insert namecardiac actin promoter
SpeciesX. laevis (frog)
Insert Size (bp)4270
/ Fusion Protein
- GFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer Sp6 (Common Sequencing Primers)
Terms and Licenses
Constructed by Enrique Amaya.
Note for Use:
Generated by inserting the 1Kb HindIII-NotI fragment from pCSGFP2 into HindIII-NotI digested pCarG.
This GFP constuct has been modified by Jim Haseloff and Jonathan Pines to be brighter and more soluble that wild-type GFP.
Note: Uniques sites are underlined in Author's Map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M12 pCarGFP2 was a gift from Randall Moon (Addgene plasmid # 17149 ; http://n2t.net/addgene:17149 ; RRID:Addgene_17149)
Map uploaded by the depositor.