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MSCV-BCL6-IRES-GFP
(Plasmid #31391)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pMSCV
  • Backbone size w/o insert (bp) 6700
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    none

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    B-cell lymphoma 6
  • Alt name
    BCL6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2200
  • GenBank ID
    NM_001706
  • Entrez Gene
    BCL6 (a.k.a. BCL5, BCL6A, LAZ3, ZBTB27, ZNF51)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-BCL6-IRES-GFP was a gift from Hilda Ye (Addgene plasmid # 31391)
  • For your References section:

    BCL-6 negatively regulates macrophage proliferation by suppressing autocrine IL-6 production. Yu RY, Wang X, Pixley FJ, Yu JJ, Dent AL, Broxmeyer HE, Stanley ER, Ye BH. Blood. 2005 Feb 15. 105(4):1777-84. 10.1182/blood-2004-08-3171 PubMed 15507530