|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40339||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerDavid A. Williams (PMID: 10961859)
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Alt nameB-cell CLL/lymphoma 6
SpeciesH. sapiens (human)
Mutationoptimized translation initiation codon
/ Fusion Protein
- IRES eGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer pLXSN_5
- 3′ sequencing primer IRES-R2 (gacggcaatatggtggaaa) (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byHuman BCL-6 cDNA with optimized translation initiation codon obtained from L.M. Staudt, NCI, NIH.
Terms and Licenses
The pMIEG3 plasmid was obtained from D.A. Williams (Indiana University School of Medicine). pMIEG3 is a murine stem cell virus (MSCV) retrovirus construct in which the gene encoding GFP is driven by the MSCV long terminal repeat. pMIEG3 contains an IRES that allows coexpression of the GFP gene and a second gene. The human BCL-6 cDNA with an optimized translation initiation codon (obtained from L.M. Staudt, NCI, NIH) was cloned into pMIEG3 via NotI and XhoI restriction sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMIEG3-BCL6 was a gift from Alexander Dent (Addgene plasmid # 40339 ; http://n2t.net/addgene:40339 ; RRID:Addgene_40339)
For your References section:BCL-6 regulates chemokine gene transcription in macrophages. Toney LM, Cattoretti G, Graf JA, Merghoub T, Pandolfi PP, Dalla-Favera R, Ye BH, Dent AL. Nat Immunol. 2000 Sep;1(3):214-20. 10.1038/79749 PubMed 10973278