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pMIEG3-BCL6
(Plasmid #40339)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40339 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMIEG3
  • Backbone manufacturer
    David A. Williams (PMID: 10961859)
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BCL6
  • Alt name
    BCL-6
  • Alt name
    B-cell CLL/lymphoma 6
  • Species
    H. sapiens (human)
  • Mutation
    optimized translation initiation codon
  • GenBank ID
    NG_007149.1
  • Tag / Fusion Protein
    • IRES eGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer pLXSN_5
  • 3′ sequencing primer IRES-R2 (gacggcaatatggtggaaa)
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The pMIEG3 plasmid was obtained from D.A. Williams (Indiana University School of Medicine). pMIEG3 is a murine stem cell virus (MSCV) retrovirus construct in which the gene encoding GFP is driven by the MSCV long terminal repeat. pMIEG3 contains an IRES that allows coexpression of the GFP gene and a second gene. The human BCL-6 cDNA with an optimized translation initiation codon (obtained from L.M. Staudt, NCI, NIH) was cloned into pMIEG3 via NotI and XhoI restriction sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIEG3-BCL6 was a gift from Alexander Dent (Addgene plasmid # 40339 ; http://n2t.net/addgene:40339 ; RRID:Addgene_40339)
  • For your References section:

    BCL-6 regulates chemokine gene transcription in macrophages. Toney LM, Cattoretti G, Graf JA, Merghoub T, Pandolfi PP, Dalla-Favera R, Ye BH, Dent AL. Nat Immunol. 2000 Sep;1(3):214-20. 10.1038/79749 PubMed 10973278