BCL6-H2Kk-RV (H2K-BCL6)
(Plasmid #40352)

• Depositing Lab: Alexander Dent
• Publication: Mondal et al J Immunol. 2010 Apr 15;184(8):4123-32. Epub 2010 Mar 8.

Backbone

• Vector backbone: MSCV2.1-H2Kk
• Backbone manufacturer: Mark Kaplan
• Modifications to backbone: Mouse H2K-k cDNA cloned after MSCV2.1 IRES
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BCL6
• Alt name: BCL-6
• Alt name: B-cell CLL/lymphoma 6
• Species: H. sapiens (human)
• GenBank ID: NG_007149.1
• Entrez Gene: BCL6 (a.k.a. BCL5, BCL6A, LAZ3, ZBTB27, ZNF51)
• Tag / Fusion Protein:
  • IRES mouse H2Kk (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII/BamHI (destroyed during cloning)
• 3′ cloning site: BamHI/BglII (destroyed during cloning)
• 5′ sequencing primer: pLXSN_5
• 3′ sequencing primer: IRES-R2 (gacggcaatatggtggaaa)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A MSCV2.1 retroviral vector was modified by cloning of mouse H2K-k cDNA after the IRES sequence to make MSCV2.1-H2Kk. MSCV2.1-H2Kk was obtained from Dr. Mark Kaplan. MSCV2.1-H2Kk was then cut with BglII and a human BCL6 cDNA with BamHI ends was cloned into the BglII site.