- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression, Cre/Lox ; phiC31/attP
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1600
/ Fusion Protein
- ECFP (N terminal on backbone)
- Cloning method Gateway Cloning
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
Docking site for phiC31 mediated single copy integration. Additional loxP site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDOCKING-Neo was a gift from Gerhart Ryffel (Addgene plasmid # 31441)
For your References section:Double conditional human embryonic kidney cell line based on FLP and PhiC31 mediated transgene integration. Waldner C, Rempel O, Schutte F, Yanik M, Solomentsew N, Ryffel GU. BMC Res Notes. 2011 Oct 18;4:420. 10.1186/1756-0500-4-420 PubMed 22008483
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.