Addgene - pDOCKING-Neo Plasmid Data

Plasmid 31441: pDOCKING-Neo

Gene/insert name:
ECFP-neomycin
Insert size:
1600
Fusion protein or tag:
ECFP
Terminal:
N terminal on backbone
Vector backbone:
pCSGFP2
(Search Vector Database)
Vector type:
Mammalian Expression, Cre/Lox ; phiC31/attP
Backbone size w/o insert (bp):
4000
Cloning method:
Gateway Cloning
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Gerhart Ryffel
Terms and Licenses:
MTA

Comments:

Docking site for phiC31 mediated single copy integration. Additional loxP site.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Double conditional human embryonic kidney cell line based on FLP and PhiC31 mediated transgene integration. Waldner et al (BMC Res Notes. 2011 Oct 18;4:420. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31441" in your Materials and Methods section.