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pDOCKING-Neo
(Plasmid #31441)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCSGFP2
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression, Cre/Lox ; phiC31/attP
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    ECFP-neomycin
  • Insert Size (bp)
    1600
  • Tag / Fusion Protein
    • ECFP (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Docking site for phiC31 mediated single copy integration. Additional loxP site.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDOCKING-Neo was a gift from Gerhart Ryffel (Addgene plasmid # 31441)
  • For your References section:

    Double conditional human embryonic kidney cell line based on FLP and PhiC31 mediated transgene integration. Waldner C, Rempel O, Schutte F, Yanik M, Solomentsew N, Ryffel GU. BMC Res Notes. 2011 Oct 18;4:420. 10.1186/1756-0500-4-420 PubMed 22008483