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pMXs-IP GFP-NBR1
(Plasmid #38283)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 38283 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMXs-IP
  • Backbone manufacturer
    Dr. Toshio Kitamura of the University of Tokyo
  • Backbone size w/o insert (bp) 5800
  • Total vector size (bp) 6800
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Next to BRCA1 gene 1 protein
  • Alt name
    NBR1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2900
  • GenBank ID
    D30756
  • Entrez Gene
    NBR1 (a.k.a. 1A1-3B, IAI3B, M17S2, MIG19)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (destroyed during cloning)
  • 3′ cloning site Bgl II (destroyed during cloning)
  • 5′ sequencing primer CGACCACTACCAGCAGAACA
  • 3′ sequencing primer GAGGAACTGCTTCCTTCACG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

DNA res. 1, 223-229, (1994)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs-IP GFP-NBR1 was a gift from Noboru Mizushima (Addgene plasmid # 38283 ; http://n2t.net/addgene:38283 ; RRID:Addgene_38283)
  • For your References section:

    p62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. Itakura E, Mizushima N. J Cell Biol. 2011 Jan 10;192(1):17-27. 10.1083/jcb.201009067 PubMed 21220506