Addgene - pSLIK sh human Rb 1534 hyg Plasmid Data

Plasmid 31500: pSLIK sh human Rb 1534 hyg

Gene/insert name:
RB shRNA
Alt name:
RB1
Species:
H. sapiens (human)
Entrez Gene:
RB1 (RP11-174I10.1, OSRC, RB, p105-Rb, pRb, pp110)
Fusion protein or tag:
EGFP
Terminal:
N terminal on backbone
Mutation:
The target sequence for shRB 1534 is GAACGATTATCCATTCAAA
Vector backbone:
pSLIK-EGFP
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size w/o insert (bp):
13700
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
EGFP-C
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3
High or low copy:
Unknown
Selectable markers:
Hygromycin
Sequence: View sequences (2)
Principal Investigator:
Julien Sage
Terms and Licenses:
MTA

Comments:

The whole sequence for the shRNA including the loop should be from 3872-3931 in the vector: AGCGAAACGATTATCCATTCAAATAGTGAAGCCACAGATGTATTTGAAT

The shRNA is downstream of the EGFP
GGATAATCGTT

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31500" in your Materials and Methods section.