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pKin1A
(Plasmid #31607)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31607 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1 (EGFP removed)
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3940
  • Modifications to backbone
    Deleted EGFP (601-1390) from original pEGFP-C1 vector
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E. coli DH5α, 16hrs at 37C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    kinesin-1A
  • Alt name
    KIF5A
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3564
  • GenBank ID
    BC058396
  • Entrez Gene
    Kif5a (a.k.a. D10Bwg0738e, Khc, Kif5, Kns, mKIAA4086)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer specific sequence primer
  • 3′ sequencing primer specific sequence primer
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Kinesin-1A cDNA (GenBank accession BC058396, I.M.A.G.E. clone:6824963, MGC:67074) was obtained from American Type Culture Collection (Manassas, VA)
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKin1A was a gift from Anthony Brown (Addgene plasmid # 31607)
  • For your References section:

    Tight functional coupling of kinesin-1A and dynein motors in the bidirectional transport of neurofilaments. Uchida A, Alami NH, Brown A. Mol Biol Cell. 2009 Dec . 20(23):4997-5006. 10.1091/mbc.E09-04-0304 PubMed 19812246