Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31816||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6300
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
MutationT219A and Y221F
Entrez GeneMapk7 (a.k.a. BMK-1, BMK1, ERK, ERK-5, ERK5, Erk5-, Erk5-T, PRKM7, b2b2346C, b2b2346Clo)
- Promoter PCMV-LTR
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer MSCV primer
- 3′ sequencing primer MSCV reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
ERK5-AEF was added by shuttling the NheI(blunted)-NotI cDNA fragments from a previously described pCI plasmid construct (Kasler et al, 2000) into pBS-IRES and then cloning the NotI fragments into the NotI site of the MSCV-puro vector.
Addgene sequencing results found an additional L->V point mutation at residue 188. This is a conservative change and depositor says this should not alter the function of this construct.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MSCV-ERK5-AEF was a gift from Astar Winoto (Addgene plasmid # 31816 ; http://n2t.net/addgene:31816 ; RRID:Addgene_31816)
For your References section:Transcriptional regulation of tissue-specific genes by the ERK5 mitogen-activated protein kinase. Sohn SJ, Li D, Lee LK, Winoto A. Mol Cell Biol. 2005 Oct;25(19):8553-66. 10.1128/MCB.25.19.8553-8566.2005 PubMed 16166637