|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25997||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6543
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameCre recombinase
Insert Size (bp)1053
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site NsiI (not destroyed)
- 5′ sequencing primer CCTTCACCGAGGGCCTATTTC (Common Sequencing Primers)
Second AgeI site is introduced preventing AgeI-EcoRI shRNA subcloning. TRC library shRNAs can be subcloned as an AccI fragment.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LV-Cre pLKO.1 was a gift from Elaine Fuchs (Addgene plasmid # 25997 ; http://n2t.net/addgene:25997 ; RRID:Addgene_25997)
For your References section:Rapid functional dissection of genetic networks via tissue-specific transduction and RNAi in mouse embryos. Beronja S, Livshits G, Williams S, Fuchs E. Nat Med. 2010 Jul . 16(7):821-7. 10.1038/nm.2167 PubMed 20526348