Purposea phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31855||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Insert Size (bp)948
MutationS13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T and N290Y relative to RpBphP2
- Promoter araBAD
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pBAD_fwd_primer (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
For additional protocols on using iRFP in other tissue types please review:
Verkhusha. Deep-tissue photoacoustic tomography of a genetically encoded near-infrared fluorescent probe. Angewandte Chemie Int. Ed. 2012, 51: 1448-1451.
For more information on in vivo imaging, please see:
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBAD/His-B-iRFP was a gift from Vladislav Verkhusha (Addgene plasmid # 31855 ; http://n2t.net/addgene:31855 ; RRID:Addgene_31855)
For your References section:Bright and stable near-infrared fluorescent protein for in vivo imaging. Filonov GS, Piatkevich KD, Ting LM, Zhang J, Kim K, Verkhusha VV. Nat Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918. 10.1038/nbt.1918 PubMed 21765402