Addgene - piRFP Plasmid Data

Plasmid 31857: piRFP

Gene/insert name:
iRFP
Insert size:
948
Species:
Rhodopseudomonas palustris
GenBank ID:
JN247409
Mutation:
T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T and N290Y relative to RpBphP2
Vector backbone:
pEGFP-C1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Promoter:
CMV
Cloning site 5':
NheI
Site destroyed during cloning:
Yes
Cloning site 3':
KpnI
Site destroyed during cloning:
Yes
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
SV40pA-R
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Supplemental document:
Verkhusha lab feature article (application/pdf)
Principal Investigator:
Vladislav Verkhusha
Terms and Licenses:
MTA

Comments:

piRFP was derived from pEGFP-C1, whereby iRFP was inserted in place of EGFP. The iRFP in this plasmid has a stop codon at its end making it unsuitable for generating fusions. This plasmid can be used for cytoplasmic expression of iRFP or as a source of the iRFP gene.

For additional protocols on using iRFP in other tissue types please review:
Verkhusha. Deep-tissue photoacoustic tomography of a genetically encoded near-infrared fluorescent probe. Angewandte Chemie Int. Ed. 2012, 51: 1448-1451.
PubMed: http://www.ncbi.nlm.nih.gov/pubmed/22213541/

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Bright and stable near-infrared fluorescent protein for in vivo imaging. Filonov et al (Nat Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31857" in your Materials and Methods section.