|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31860||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6273
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namenMyc mutant-1 3'UTR
SpeciesM. musculus (mouse)
Insert Size (bp)900
MutationSecond Let-7c Seed changed from TACCTC to TAACGC
- Promoter SV40
/ Fusion Protein
- Luciferase (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer psiCHECK2-F (Common Sequencing Primers)
Please note that there are 3 additional point mutations (in addition to the mutated seed sequence). The additional mutations should not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:psiCHECK-2-nMyc-m2_3'UTR was a gift from Robert Blelloch (Addgene plasmid # 31860 ; http://n2t.net/addgene:31860 ; RRID:Addgene_31860)
For your References section:Opposing microRNA families regulate self-renewal in mouse embryonic stem cells. Melton C, Judson RL, Blelloch R. Nature. 2010 Feb 4;463(7281):621-6. Epub 2010 Jan 6. 10.1038/nature08725 PubMed 20054295