PurposeHuman OCT4 genomic targeting donor
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31938||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 8012
Vector typetargeting donor
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GenePOU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
/ Fusion Protein
- eGFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site - (unknown if destroyed)
- 3′ cloning site - (unknown if destroyed)
- 5′ sequencing primer M13
- 3′ sequencing primer M13 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that the Puromycin selection cassette in this plasmid has a R166H mutation. This mutation has been observed in other plasmids and should not have any affect on the function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:OCT4-2A-eGFP-PGK-Puro was a gift from Rudolf Jaenisch (Addgene plasmid # 31938 ; http://n2t.net/addgene:31938 ; RRID:Addgene_31938)
For your References section:Genetic engineering of human pluripotent cells using TALE nucleases. Hockemeyer D, Wang H, Kiani S, Lai CS, Gao Q, Cassady JP, Cost GJ, Zhang L, Santiago Y, Miller JC, Zeitler B, Cherone JM, Meng X, Hinkley SJ, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R. Nat Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927. 10.1038/nbt.1927 PubMed 21738127