Addgene - OCT4-2A-eGFP-PGK-Puro Plasmid Data

Plasmid 31938: OCT4-2A-eGFP-PGK-Puro

Gene/insert name:
OCT4
Alt name:
Pou5F1
Species:
H. sapiens (human)
Entrez Gene:
POU5F1 (DADB-104B20.2, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)
Fusion protein or tag:
eGFP
Terminal:
C terminal on insert
Vector backbone:
PCR2.1
(Search Vector Database)
Backbone manufacturer:
invitrogen
Vector type:
targeting donor
Backbone size w/o insert (bp):
8012
Cloning site 5':
-
Site destroyed during cloning:
Unknown
Cloning site 3':
-
Site destroyed during cloning:
Unknown
5' sequencing primer:
M13 List of Sequencing Primers
3' sequencing primer:
M13
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Top10/P3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers, other:
Puro
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Rudolf Jaenisch
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Genetic engineering of human pluripotent cells using TALE nucleases. Hockemeyer et al (Nat Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31938" in your Materials and Methods section.