Addgene - pLKO.1p shDna2 Plasmid Data

Plasmid 31951: pLKO.1p shDna2

Gene/insert name:
shRNA against hDna2
Alt name:
Dna2L
shRNA sequence:
5’-CATAGCCAGTAGTATTCGATG-3’
Species:
H. sapiens (human)
GenBank ID:
NM_001080449
Entrez Gene:
DNA2 (RP11-9E13.1, DNA2L, PEOA6, hDNA2)
Vector backbone:
pLKO.1 puro
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size w/o insert (bp):
7200
Promoter:
U6
Cloning site 5':
Age1
Site destroyed during cloning:
No
Cloning site 3':
EcoR1
Site destroyed during cloning:
No
5' sequencing primer:
NA List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Principal Investigator:
Sheila Stewart
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Human Dna2 is a nuclear and mitochondrial DNA maintenance protein. Duxin et al (Mol Cell Biol. 2009 Aug;29(15):4274-82. Epub 2009 Jun 1. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31951" in your Materials and Methods section.