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pLKO.1p shDna2
(Plasmid #31951)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 31951 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
    pLKO.1 puro
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
    shRNA against hDna2
  • Alt name
  • gRNA/shRNA sequence
  • Species
    H. sapiens (human)
  • GenBank ID
  • Entrez Gene
    DNA2 (a.k.a. RP11-9E13.1, DNA2L, hDNA2)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Age1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer NA
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.1p shDna2 was a gift from Sheila Stewart (Addgene plasmid # 31951)
  • For your References section:

    Human Dna2 is a nuclear and mitochondrial DNA maintenance protein. Duxin JP, Dao B, Martinsson P, Rajala N, Guittat L, Campbell JL, Spelbrink JN, Stewart SA. Mol Cell Biol. 2009 Aug;29(15):4274-82. Epub 2009 Jun 1. 10.1128/MCB.01834-08 PubMed 19487465