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pLKO.1p shDna2
(Plasmid #31951)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pLKO.1 puro
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    shRNA against hDna2
  • Alt name
    Dna2L
  • gRNA/shRNA sequence
    5’-CATAGCCAGTAGTATTCGATG-3’
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_001080449
  • Entrez Gene
    DNA2 (a.k.a. RP11-9E13.1, DNA2L, hDNA2)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Age1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer NA
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.1p shDna2 was a gift from Sheila Stewart (Addgene plasmid # 31951)
  • For your References section:

    Human Dna2 is a nuclear and mitochondrial DNA maintenance protein. Duxin JP, Dao B, Martinsson P, Rajala N, Guittat L, Campbell JL, Spelbrink JN, Stewart SA. Mol Cell Biol. 2009 Aug;29(15):4274-82. Epub 2009 Jun 1. 10.1128/MCB.01834-08 PubMed 19487465