Gene/insert name: DNMT3B
Alt name: PDB: 3QKJ
Alt name: DNA (cytosine-5-)-methyltransferase 3 beta, PWWP domain
Insert size: 450
Species:
H. sapiens (human)
Entrez Gene:
DNMT3B
(RP5-1085F17.2, ICF, ICF1, M.HsaIIIB)
Fusion protein or tag: His (with TEV cleavage)
Terminal: N terminal on backbone
Mutation: PWWP domain only (amino acids 293-442)
Vector backbone:
pET28-MHLSearch Vector Database )
Backbone manufacturer:
Structural Genomics Consortium (Addgene plasmid 26096)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
7325
Cloning method: Ligation Independent Cloning
5' sequencing primer:
T7
List of Sequencing Primers
3' sequencing primer:
T7-term
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence:
View sequences (3)
Principal Investigator:
Cheryl Arrowsmith
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
32044" in your Materials and Methods section.
PDB: 3QKJ. http://www.thesgc.org/structures/details?pdbid=...