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pJW1
(Plasmid #32130)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BW25142 (a lambda pir strain)
  • Growth instructions
    For information on growth strain: (http://cgsc.biology.yale.edu/Strain.php?ID=70715)
  • Copy number
    High Copy

Sequence Information

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJW1 was a gift from Eric Rubin (Addgene plasmid # 32130)
  • For your References section:

    Depletion of antibiotic targets has widely varying effects on growth. Wei JR, Krishnamoorthy V, Murphy K, Kim JH, Schnappinger D, Alber T, Sassetti CM, Rhee KY, Rubin EJ. Proc Natl Acad Sci U S A. 2011 Mar 8;108(10):4176-81. Epub 2011 Feb 22. 10.1073/pnas.1018301108 PubMed 21368134