Addgene - pJFRC150-20XUAS-IVS-Flp1::PEST Plasmid Data

Plasmid 32132: pJFRC150-20XUAS-IVS-Flp1::PEST

Gene/insert name:
Flippase1::PEST
Species:
S. cerevisiae (budding yeast)
Fusion protein or tag:
PEST
Terminal:
C terminal on insert
Mutation:
Aspartic Acid at Amino Acid 5
Vector backbone:
pJFRC7-20XUAS-IVS-mCD8::GFP
(Search Vector Database)
Vector type:
Insect Expression
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
hsp70 F: GAGCGCCGGAGTATAAATAGAG List of Sequencing Primers
3' sequencing primer:
SV40 R: CCATTCATCAGTTCCATAGG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Top10/P3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Gerald Rubin
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Multiple new site-specific recombinases for use in manipulating animal genomes. Nern et al (Proc Natl Acad Sci U S A. 2011 Aug 9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32132" in your Materials and Methods section.