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pAd FLAG HDAC5 S259A/S498A
(Plasmid #32208)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pAd CMV/V5 DEST
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 37000
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    HDAC5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3400
  • Mutation
    S259A/S498A
  • GenBank ID
    NM_001015053
  • Entrez Gene
    HDAC5 (a.k.a. HD5, NY-CO-9)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Subcloned and converted into Gateway from original plasmid described previously in Berdeux et al. PMID 17468767
  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAd FLAG HDAC5 S259A/S498A was a gift from Reuben Shaw (Addgene plasmid # 32208)
  • For your References section:

    Class IIa histone deacetylases are hormone-activated regulators of FOXO and mammalian glucose homeostasis. Mihaylova MM, Vasquez DS, Ravnskjaer K, Denechaud PD, Yu RT, Alvarez JG, Downes M, Evans RM, Montminy M, Shaw RJ. Cell. 2011 May 13;145(4):607-21. 10.1016/j.cell.2011.03.043 PubMed 21565617