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pGL3Basic-miR126-EGFL7-Promoter
(Plasmid #32244)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pGL3Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    miR-126 Promoter 5' UTR
  • Alt name
    EGFL7 Promoter 5' UTR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1770
  • Mutation
    Promoter region 5' UTR
  • Promoter 5' UTR

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GCCTGCTGCCAACTTGTTCT
  • 3′ sequencing primer ATGGACCCTAGCCCTTGCTG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3Basic-miR126-EGFL7-Promoter was a gift from Charles Lowenstein (Addgene plasmid # 32244)
  • For your References section:

    Ets-1 and Ets-2 regulate the expression of microRNA-126 in endothelial cells. Harris TA, Yamakuchi M, Kondo M, Oettgen P, Lowenstein CJ. Arterioscler Thromb Vasc Biol. 2010 Oct;30(10):1990-7. Epub 2010 Jul 29. 10.1161/ATVBAHA.110.211706 PubMed 20671229