Addgene - pGL3Basic-miR126-EGFL7-Promoter Plasmid Data

Plasmid 32244: pGL3Basic-miR126-EGFL7-Promoter

Gene/insert name:
miR-126 Promoter 5' UTR
Alt name:
EGFL7 Promoter 5' UTR
Insert size:
1770
Species:
H. sapiens (human)
Mutation:
Promoter region 5' UTR
Vector backbone:
pGL3Basic
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
4818
Promoter:
5' UTR
Cloning method:
Ligation Independent Cloning
5' sequencing primer:
GCCTGCTGCCAACTTGTTCT List of Sequencing Primers
3' sequencing primer:
ATGGACCCTAGCCCTTGCTG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Charles Lowenstein
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Ets-1 and Ets-2 regulate the expression of microRNA-126 in endothelial cells. Harris et al (Arterioscler Thromb Vasc Biol. 2010 Oct;30(10):1990-7. Epub 2010 Jul 29. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32244" in your Materials and Methods section.