|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11955||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6600
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1800
Entrez Genecre (a.k.a. P1_gp003)
- Cloning method Restriction Enzyme
- 5′ sequencing primer EF1a-Fwd (Common Sequencing Primers)
Terms and Licenses
Article Citing this Plasmid
pBS505 is similar to pBS598 except the NcoI site within EGFPcre is replaced by a cluster of 6 bp cutters (BspE1, BglII, XhoI, SacI, HindIII, EcoRI, PstI, SalI, NotI). pBS598 carries the EGFPcre fusion gene under the control of the strong elongation factor EF1alpha promoter. This promoter expresses at high levels in most cell types, including embryonic stem (ES) cells. However its use in transgenic mice is not recommended: the promoter is often silenced in a transgene setting. The GFP moiety of the fusion gene carries the S65T mutation for enhanced fluorescence and is codon-optimised for higher level expression.
Note: pBS513 (EF1alpha-cre) is very similar to pBS598 but carries the wt cre gene and thus serves as the non-fluorescent counterpart of pBS598.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS505 EF1alpha-EGFPcre* was a gift from Brian Sauer (Addgene plasmid # 11955 ; http://n2t.net/addgene:11955 ; RRID:Addgene_11955)
For your References section:GFPcre fusion vectors with enhanced expression. Le Y, Miller JL, Sauer B. Anal Biochem. 1999 Jun 1. 270(2):334-6. 10.1006/abio.1999.4110 PubMed 10334853