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hATMS1981A
(Plasmid #32300)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32300 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Growth instructions
    see attachment
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ATM
  • Alt name
    ATI
  • Alt name
    ATA
  • Alt name
    ATDC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    9581
  • Mutation
    S1981A
  • Entrez Gene
    ATM (a.k.a. AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1)
  • Tags / Fusion Proteins
    • Flag (N terminal on backbone)
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI-SAl I (destroyed during cloning)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGHrev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hATMS1981A was a gift from Michael Kastan (Addgene plasmid # 32300 ; http://n2t.net/addgene:32300 ; RRID:Addgene_32300)
  • For your References section:

    DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation. Bakkenist CJ, Kastan MB. Nature. 2003 Jan 30;421(6922):499-506. 10.1038/nature01368 PubMed 12556884