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PurposeLive imaging of apoptotic cells in zebrafish
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 32359 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBH-UAS-gtwy-YFP
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Backbone manufacturerMichael L. Nonet
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Vector typeZebrafish expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namesecreted human annexin 5
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SpeciesH. sapiens (human)
- Promoter UAS
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer cctcgcccttgctcaccat (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe backbone plasmid (pBH-UAS-gtwy-YFP) was created by the Michael L. Nonet lab (Wustl).
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Article Citing this Plasmid
Depositor Comments
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBH-UAS-secA5-YFP was a gift from Randall Peterson (Addgene plasmid # 32359 ; http://n2t.net/addgene:32359 ; RRID:Addgene_32359) -
For your References section:
Live imaging of apoptotic cells in zebrafish. van Ham TJ, Mapes J, Kokel D, Peterson RT. FASEB J. 2010 Nov;24(11):4336-42. Epub 2010 Jul 2. 10.1096/fj.10-161018 PubMed 20601526