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pGL3Basic-Mdm2-T4
(Plasmid #32370)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pGL3Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Mdm2 promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    244
  • Entrez Gene
    Mdm2 (a.k.a. 1700007J15Rik, AA415488, Mdm-2)

Cloning Information

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3Basic-Mdm2-T4 was a gift from Hong Wu (Addgene plasmid # 32370)
  • For your References section:

    PTEN regulates Mdm2 expression through the P1 promoter. Chang CJ, Freeman DJ, Wu H. J Biol Chem. 2004 Jul 9;279(28):29841-8. Epub 2004 Apr 16. 10.1074/jbc.M401488200 PubMed 15090541